1. "weaning reaction" is described as a #physiological CD4+ T cell response to to #microbiota+solid #foods in mouse pups that occurs between 2-4 weeks after birth measured by analysis of #TNF-alpha and #IFN-gamma levels. It does not occur in #germfree pups devoid of microbiota. pic.twitter.com/pSGWXOUBoC— David Usharauli (@3DiMMUNE) March 21, 2019
3. More importantly, however, #microbiota exposure between 2-4 weeks after birth but not later could restore #physiological "#weaning reaction" in T cells in #germfree pups. #tolerance #immunology pic.twitter.com/9KUtk3MT7R— David Usharauli (@3DiMMUNE) March 21, 2019
5. Interestingly, "weaning reaction" in #newborn pups were dependent on presence of gut residing RORgt+ FOXP3+ T regulatory cells (Tregs). #tolerance pic.twitter.com/s1nq2HPShK— David Usharauli (@3DiMMUNE) March 21, 2019
2nd Correction (#6): giving #SCFAs to #antibiotic-treated newborn pups could prevent #inflammation later. It appears this effect of SCFAs requires #Tregs and #microbiota (it does not work in GF pups) that provides #antigens to Tregs to keeps them in #functioning state. pic.twitter.com/2ewMoDRobh— David Usharauli (@3DiMMUNE) March 21, 2019
This suggests that the effect of SCFAs is mediated via Tregs and that artifically generated "weaning reaction" with SCFAs without proper Treg contribution (driven by microbiota and their antigens) is not optimal.— David Usharauli (@3DiMMUNE) March 21, 2019
